Detection of equine arteritis virus in the semen of carrier stallions by using a sensitive nested PCR assay.
نویسندگان
چکیده
A nested PCR, developed for the detection of equine arteritis virus (EAV) in semen, detected less than 2.5 PFU of EAV per ml of naturally infected seminal plasma. Based on results from testing 88 semen samples from 70 stallions, the sensitivity and specificity of the test were 100 and 97%, respectively.
منابع مشابه
Rt-pcr as Effective Detection Method of Equine Arteritis Virus in the Semen of Naturally Infected Stallions
The aim of the study was to present the usefulness of one tube reverse transcriptase polymerase chain reaction assay (RT-PCR) in comparison to virus isolation test (VI) in the detection of equine arteritis virus. The group of 82 semen samples taken from different stallions was examined. Equine arteritis virus was isolated in RK-13 cell monolayers from the seminal plasma of 11 animals. The same ...
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Routine detection of equine arteritis virus (EAV) can be achieved by virus isolation (VI) in cell culture, or by the amplification of viral genome by molecular methods. To simplify molecular diagnosis, a number of different Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and RT-nested PCR (RT-nPCR) assays were compared, and a one-tube method was developed and optimised utilizing a fluo...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 35 8 شماره
صفحات -
تاریخ انتشار 1997